3) How will we know if our species produces a citrate...

3) How will we know if our species produces a citrate permease? When determining if a bacterial species generates citrate permease, an enzyme that permits the transfer of citrate molecules into cells, the citrate test is a useful technique in microbiology.

Transcript

00:01 Species of bacteria produce a citrate permease? so first of all, we want to know what the function of citrate permease is.

00:12 We know that the citrate permease is able to transport the citrate into the bacteria cell so they can be used as an energy source.

00:38 So in this experiment, the citrate will be broken down into acetyl -coa and oxaloacetate oxaloacetate and then they are being metabolized and produce a basic product which is the sodium carbonate so citrate is being used as energy source and it produce a metabolite which is sodium carbonate and this is a basic metabolite so from there, we are able to use certain agar plates that contain citrate and also a ph indicator to show if the citrate is being metabolized.

01:26 So the very first step, you are going to make a medium that contains the citrate agar, which is the sole carbon source.

01:42 Let's say this is called the simons citrate agar plates.

01:55 So in this particular agar plate, citrate is used as a main only carbon source.

02:03 Second, you want to make sure that the citrate agar plate includes a ph indicator such as bromothymol blue.

02:15 You have a ph indicator such as bromothymol blue.

02:34 Blue.

02:36 It will turn blue when it sits in a higher ph.

02:40 Now once you have the bromothemal blue as indicator, you want to streak or inoculate the bacteria that express the citrate permeates onto the surface of a citric agar plate.

02:53 So so inoculate bacteria to the citrate plate...

Question

3) How will we know if our species produces a citrate permease? When determining if a bacterial species generates citrate permease, an enzyme that permits the transfer of citrate molecules into cells, the citrate test is a useful technique in microbiology.

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