4. The plasmid is mobilized for transfer when O a helicase unwinds the two strands. O an endonuclease cleaves it at the origin of replication. O it is glycosylated by the Golgi complex. O single-strand stabilizing proteins attach to it.
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This refers to the process of bacterial conjugation, where genetic material (often a plasmid) is transferred from a donor bacterium to a recipient bacterium. Show more…
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Identify the importance of the following features in conjugating donor bacteria. Drag the terms on the left to the appropriate blanks on the right to complete the sentences. the T strand DNA the conjugation pilus homologous recombination pilin the relaxosome relaxase the origin of transfer is the site in F plasmid DNA where binding and single-strand cleavage of F DNA occurs, and it is the site where rolling circle replication of F plasmid DNA is initiated. makes physical connection between the donor and recipient cell. is the part of F-origin DNA that is cleaved, bound, and fed into the conjugation pilus. is important for stable maintenance and expression of donor chromosomal DNA in the exconjugate cell when transfer is from an Hfr cell. is the protein component that assembles into the conjugation pilus. is the component of protein complex that binds to the 5' end of the F origin after it is cleaved and that binds to coupling protein as part of the process of feeding the DNA into the conjugation pilus. is a complex that initiates transfer of DNA by cleaving one strand of origin DNA. Its components also bind to the 5' end of the cleaved DNA and help it interact with a coupling protein, which is required for feeding the DNA strand into the conjugation pilus.
Marisa A.
During initiation of DNA replication in a single bacterial plasmid, origin recognition protein binds at a single origin of replication. DNA helicase begins unwinding dsDNA in a GC-rich region. ssDNA binding proteins bind and activate DNA polymerase. All of the above (b&c).
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Which of the following is correct concerning the initiation of bacterial replication? The DNA double helix spontaneously unwinds. The strands are initially separated at AT-rich regions of DNA. It involves a region of DNA called the origin of replication (ori). Following initial separation, the DNA helicase enzymes continue to unwind the DNA.
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