00:01
Hi, pglo is a plasmid is a plasmid which contain gfp, gfp sequence and ampicillin resistant and ampicillin resistant resistant gene which code for beta lactamase code for beta lactamase so the first one is minus pglo on lb so the first plate consists of only nutrient medium nutrient medium that is lb and no emphasis linlm lb and no emphasis lb and no no ampicillin.
00:57
So the bacterial cell grow normally on the medium and form a lawn of very, form a lawn of very dense white bacterial colony.
01:12
Second one is positive p -g -l -l -o on l -bel -bon -bel -bon -bel -b or ampicillin.
01:32
The second plate consists of ampicillin.
01:36
Second plate consists of ampicillin along with lb medium.
01:43
Along with lb medium.
01:45
So the non -transformed bacterial cell, non -transformed transformed bacterial cell will not grow on it.
01:58
Many number of transformed colony of bacterial cells will be obtained will be obtained which are white colored colonies which are white colored colony third one is minus negative pglo on lb on lb or ampicillin so the third plate consists of ampicillin third plate consists of ampicillin along with lb medium, but the bacterial cell do not contain pg -l -o -plasmid.
02:47
P -g -l -o -plasmid.
02:52
Therefore, the bacterial will unable to grow...
