00:01
Okay, so this is a question about using ecori sites to digest a bacterial plasmid.
00:09
So a bacterial plasmid, when it's not cut, should be a circular piece of dna.
00:14
So let's say this is our plasmid.
00:20
And so your question says that this plasmid has two ecori sites.
00:26
So let's say one is right here, and let's say one is right here.
00:31
So this is an ecori site, and this is an ecori site.
00:46
So when ecori is around, it's going to cut at both of these sites.
00:57
Okay, so this is a question about...
01:01
So if that happens, and let's say that these are cut, how many pieces do we have? well, we have one piece here, one, and a second piece here, two...