it is possible to use high fidelity polymerases such as pfu or tth in pcrs rather than taq most

Name: it is possible to use high fidelity polymerases such as pfu or tth in pcrs rather than taq most high fidelity polymerases however require longer than taq to complete the elongation step in p 56642
Uploaded: 2022-05-08T19:14:52-08:00
Duration: 49 s
Channel: Mystique Till
Description: it is possible to use high fidelity polymerases such as pfu or tth in pcrs rather than taq most high fidelity polymerases however require longer than taq to complete the elongation step in p 56642

Question

Mystique Till · Accepted Answer

Step 1: High-fidelity polymerases, such as Pfu or Tth, have proofreading capabilities that allow

Question

It is possible to use "high-fidelity" polymerases, such as Pfu or Tth, in PCRs, rather than Taq. Most high-fidelity polymerases, however, require longer than Taq to complete the elongation step in PCR. Why is that?

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