Lb agar with yeast extract peptone and salt is considered ...

Name: lb agar with yeast extract peptone and salt is considered select all that apply because weak it does not have many nutrients complex the exact composition of chemicals is not kn 92288
Uploaded: 2021-06-08T04:52:20-08:00
Duration: 3 min 18 s
Channel: Sylvia Puglisi
Description: lb agar with yeast extract peptone and salt is considered select all that apply because weak it does not have many nutrients complex the exact composition of chemicals is not kn 92288

LB agar (with yeast extract, peptone, and salt) is considered ____________. (select all that apply) because weak; it does not have many nutrients complex; the exact composition of chemicals is not known defined; the exact composition of chemicals is known rich; it has many nutrients differential; has a pH indicator

Transcript

00:01 In this problem, we're going to talk about a specific growth medium for bacteria called mekonki agar, and we're going to analyze the properties of this when we take away the ph indicator that is normally involved.

00:15 So first, let's talk about what mekonky agar is for.

00:19 It's used to isolate or to grow in isolation gram -negative rod -shaped bacteria from clinical specimens, particularly like urine, in order to see if certain infects.

00:30 Pathogens are in a specimen.

00:36 So what does mccongy -aggar have in order to culture those bacteria? well, first, it needs a bunch of nutrients to make sure that they have enough to eat, and one of those nutrients that's going to be important later is lactose.

00:50 Other substances found in mcconkey agar include crystal violet, which is a dye, so it's useful for dying the bacteria, but it also inhibits the growth of gram -positive bacteria.

01:02 Which is useful because we want to grow gram -negative bacteria.

01:06 Another inhibitor is bile salts.

01:08 These can be isolated from the digestive tract of an animal, and they're going to inhibit any bacteria that don't normally grow in the digestive tract.

01:16 It's a pretty inhospitable environment, unless you're evolved to grow in there.

01:22 Because we're using bile salts, and we also sometimes use like a nutrient broth or nutrient mix, this is not a precisely chemically defined substance.

01:30 This is a complex medium...

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