5. What are the four reagents used for gram staining in the correct order of use? (2 pts) 6. What would happen to the stain if you forgot to rinse the slide with alcohol with a gram-negative culture? What would happen with a gram-positive culture? (4 pts) 7. What are the types of bacteria below? Are they gram negative or gram positive? (2 pts) A B 8. Why does the order of the gram staining reagents matter? (3 pts) 9. How do Gram-positive and Gram-negative bacteria differ in cellular structure? How does this contribute to their differential staining properties? (4 pts)
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The four reagents used for gram staining in the correct order of use are: Crystal Violet (primary stain), Gram's Iodine (mordant), Alcohol or Acetone (decolorizer), and Safranin (counterstain). Show more…
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