What is the P : O ratio for electrons donated by matrix NADH? P : O ratio = Incorrect Answer What is the P : O ratio for electrons donated by succinate via FADH2? P : O ratio =
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One measure of ATP-producing efficiency via oxidative phosphorylation is the P/O ratio, which equals the number of ATP produced per 1/2 O2 consumed (i.e., per electron pair donated into the respiratory chain and passed to the terminal acceptor). To determine the P/O ratio, you must first calculate the number of H+p consumed (i.e., allowed to favorably move down the proton electrochemical gradient) per ATP, which in a mitochondrion will equal: (# of c subunits in F-type ATP synthase + 3)/3. Then, you must divide the number of H+p generated per 1/2 O2 consumed (e.g., 10 for matrix NADH) by that value. Calculate the P/O ratio for mitochondrial oxidative phosphorylation, given a c11 F-type ATP synthase powered by matrix NADH. Enter your answer (a number only, rounded to the nearest tenth) in the space provided below.
Sri K.
Intact mitochondrial were isolated, carefully washed with pH 7.0 buffer, and used to measure the P/O ratio for α-ketoglutarate. Included in your assay for the measurements were ADP, phosphate (Pi), NAD+, and malonate in an appropriate buffer. Assume that all the added compounds can get into the mitochondrial matrix and that all enzymes of the citric acid cycle are active. Note that malonate is a competitive inhibitor or succinate dehydrogenase. Under these conditions, what would be the P/O ratio for α-ketoglutarate? P/O ratio = 0.5 P/O ratio = 1.5 P/O ratio = 2.0 P/O ratio = 3.5 P/O ratio = 5.0
Adi S.
The number of molecules of inorganic phosphate incorporated into organic form per atom of oxygen consumed, termed the $P: O$ ratio, was frequently used as an index of oxidative phosphorylation. (a) What is the relation of the $P:$ O ratio to the ratio of the number of protons translocated per electron pair $\left(\mathrm{H}^{+} / 2 \mathrm{e}^{-}\right)$ and the ratio of the number of protons needed to synthesize ATP and transport it to the cytoplasm $\left(\mathrm{P} / \mathrm{H}^{+}\right) ?$ (b) What are the $P:$ O ratios for electrons donated by matrix NADH and by succinate?
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