What is the probe for the gene for the fluorescence in situ hybridization (FISH) method? loss of a chromosome segment a single-stranded DNA complementary to the gene of interest hybrid cell DNA sequence
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Step 1: FISH (fluorescence in situ hybridization) is a technique used to detect specific DNA sequences in chromosomes. Show more…
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which of the following describes the structure formed when a probe hybridizes to a gene
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The technique of hybridization can be used to detect a specific DNA sequence. Northern blotting detects a specific sequence in mRNA. A short single-stranded DNA is an oligonucleotide. A piece of DNA used to detect a specific sequence in a nucleic acid by hybridization is known as a probe.
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You determine the sequence of an isolated gene and wish to find its location in the genome. Propose a procedure using fluorescence to locate your gene of interest: Your Answer: 1. Synthesis of a single-stranded probe molecule, which is complementary to your gene and labeled with a fluorescent marker. 2. Immobilization of mitotic cells. 3. Denaturation of the chromosomes. 4. Introduction of the labeled probe. 5. Hybridization. 6. Detection of the fluorescent label. 7. Identification of the chromosome and location of the gene.
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