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What is the transformation efficiency of your competent E. coli cell? 

          What is the transformation efficiency of your competent E. coli cell?

Added by Deborah S.

Biology for AP Courses
Biology for AP Courses
Julianne Zedalis, John Eggebrecht
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What is the transformation efficiency of your competent E. coli cell?
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For the positive control, you transformed a plasmid with a known DNA concentration. This allows you to calculate the transformation efficiency for your batch of competent E. coli cells. Transformation efficiency is expressed in cfu (colony forming units) per microgram (μg) of DNA (cfu/μg). Using the parameters below, calculate the transformation efficiency for your batch of E. coli:

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After my transformation of pML104 into E. coli DH5α cells, I count 2000 colonies on my whole plate. If I added 1αl of a 5ng/αl pML104 stock to 100αl of cells, and then plated a fourth of the cell mixture (25αl), what is my transformation efficiency? Include units in your final answer.

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Transcript

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00:01 So to calculate the transformation efficiency, all you have to do is knowing the number of colonies from a positive control plate and knowing the amount of dna that you use in microgram.
00:15 So in this case, we know that the dna in this case is going to be concentration times the volume.
00:31 You have a concentration of plasmid of 0 .1 nanogram per microliter and the volume of plasmid that was transformed is 1 microliter.
00:51 So you get 0 .1 nanogram.
00:55 Usually when you do the transformation efficiency, you're talking about the transformance in microgram.
01:05 So you're going to have to convert that from from nanogram to microgram.
01:10 We know that one nanogram equals 0 .001 microgram...
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