What is TRUE about a genome that has been cut with a restriction endonuclease? Multiple Choice The genome will be cut into equally sized fragments. Each chromosome will be cut at the centromere, resulting in twice the number of fragments as there are chromosomes. The genome will be cut at certain sites recognized by the restriction endonuclease, generating DNA fragments of varying size. The genome will be cut by the restriction endonuclease, and will then reassemble randomly into a single large chromosome containing the entire genome.
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An important feature of restriction enzymes is that each enzyme only recognizes the DNA at a specific sequence of bases, known as a palindrome, and cuts the DNA strand a certain number of times. The palindromic sequence can be repeated no matter what species the enzyme is from. For example, if the GAATTC palindrome is present in the DNA, the restriction enzyme will cut all instances of that palindrome. If the DNA contains multiple repeats of the GAATTC palindrome, the enzyme will produce multiple DNA fragments randomly along the DNA strand. The number of fragments produced depends on the number of repeats. When the DNA is digested with a restriction enzyme, the sizes of the fragments that are recognized can be determined.
Dominador T.
The right cuts. Suppose that a human genomic library is prepared by exhaustive digestion of human DNA with the EcoRI restriction enzyme. Fragments averaging about $4 \mathrm{kb}$ in length would be generated. Is this procedure suitable for cloning large genes? Why or why not?
3. An important feature of restriction enzymes is that each enzyme only recognizes a specific palindrome and cuts the DNA only at that specific sequence of bases. A palindromic sequence can be repeated a number of times on a strand of DNA, and the specific restriction enzyme will cut all those palindromes, no matter what species the DNA comes from. 3A. A linear DNA molecule is represented below. The DNA is represented by one line, although in actuality, DNA has two strands. If the DNA molecule has two restriction sites, specifically two repeats of a specific palindrome sequence, A and B, for a specific restriction enzyme, How many fragments would be produced if the DNA is cut by that enzyme? Number each fragment. Which fragment would be the largest? Which fragment would be the smallest? The sum of the lengths of DNA fragments 1-3 equal the total original length? True / False
Jenny W.
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