00:07
With the salmonella is it first stimulates the membrane in order to promote invasion and then suppresses the membrane once the invasion is complete.
00:18
So there is another set of proteins.
00:22
There's actually two proteins that will remediate this process by having their proteins being injected.
00:40
Sope, and that's the one that promotes that helps the membrane invasion to happen, and then spt, capital p, spt, capital p, which then blocks the effects of sope.
00:56
And so that would then sort of be the end part of it when it suppresses the membrane, roughly, is what they call it, ru -f -l -i -h -e, once the invasion is complete.
01:07
So the question is, first of all, how does the sope and the sptp affect the monomeric gtpas, which is called capital rac, in its active form is what is going to be promoting for the membrane roughly.
01:32
How does this affect the rac activity? and then another question it says in here is, how do you suppose the effects of the s -o -p -e and the s -p -t -p are staggered in time? how does that take place that they are staggered? there is one that's happening at one point, and then later on in time there's another thing that's happening.
01:58
But the answer to the first question, the s -o -p -e is what's going to act? and get the rac to get started.
02:10
And so what we're going to have to have happen here is that because gdp disassociates really pretty slowly, we're going to have some help in getting that gdp to be disassociated from gtpas.
02:35
And then instead of the gdp being there, we'll have gtp.
02:39
And the binding of the gtp in place of the gdp is what's the activation.
02:50
It stabilizes the gtp interaction and then allows for basically the guarani and exchange, if you will, and it activates the gtpas in order for the ric to do the member and ruffling...