What would happen to your membrane if we did not store it in PBS?It would dry out and the western blot would not workThe proteins would fall off
Added by Mackenzie O.
Step 1
PBS helps maintain the pH and osmotic balance, preventing the membrane from drying out. Show more…
Show all steps
Your feedback will help us improve your experience
Josee Pacheco and 56 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Why is PBS used throughout the dissection and staining procedure of Drosophila cells?
Josee P.
1) Which percent acrylamide solution would be best for the separation of smaller molecular weight proteins? a. 7.5% b. 15% c. 2% d. It should not matter since the polyacrylamide gel system separates based on charge. 2) The Western Blot technique: a. separates DNA fragments by size b. can be performed for protein or DNA isolation c. uses DNA probes to detect proteins d. allows researchers to detect changes in a protein of interest 3) After cell lysis centrifugation, which components do you expect to find in the supernatant? a. insoluble proteins and cellular components b. cellular organelles c. solubilized proteins d. insoluble proteins, cellular components, and organelles. 4) In addition to convenience, why is it common to purchase pre-poured polyacrylamide gels? (Briefly explain)
Sri K.
What would happen in the FRAP experiment if proteins did not move in the plan of a membrane?
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD