00:01
Hello students, in this video i will be answering three questions.
00:05
In the first question, we need to select an option that represents a wrong statement about rna polymerases.
00:14
The first statement is true because rna polymerase require divalent cations such as mg2 plus ions for their activity.
00:23
These ions are involved in stabilizing the rna polymerase dna complex and catalyzing the formation of phosphodiester bond between the nucleotides.
00:33
The second statement is also true because the rna polymerases can unwind short stretches of dna to expose the template strand for transcription.
00:45
This process is facilitated by rna polymerases helicase activity.
00:50
The third statement is also true because rna polymerases read the dna template, template strand in 3 prime to 5 prime direction and synthesize the complementary rna strand in 5 prime to 3 prime direction.
01:04
This process is known as transcription.
01:08
The fourth statement which states they lack 3 prime, 5 prime exonuclease activity is wrong because human rna polymerase 2 is shown to be associated with 3 prime, 5 prime exonuclease activity that removes nucleotides from 5 prime monophosphates and new other nucleotides from 3 prime end of the transcripts in isolated ternary complexes.
01:35
Therefore, fourth option which states they lack 3 prime, 5 prime exonuclease activity is wrong...