00:02
Hello students, this gel feature shows the amplification of a target dna from a genome dna.
00:14
That means in this pcr reaction, genomic dna was used as campaign to amplify the target dna.
00:26
A lane 1 shows the ladder or dna marker of 100 spacepeer.
00:35
Sphere and lane 2 and lane 3 shows the pcr reactions for amplifying the target and lane 4 is a positive control lane 5 in negative what does that mean to control and positive control here the target dna must be identified this lane and negative control mean the positive control means the positive the the target dna should not be present to gel and these are the two pcr reaction to it.
01:19
Now the question asked is we have to explain the presence of each band, these white colored bands.
01:31
Each we have to explain each band from lane two and lane three.
01:38
If we go to the lane two, lane two, we go to the lane two, we will.
01:46
We don't see any band.
01:56
Although it is mentioned here that the band is very faint, but we can see where the band is.
02:05
So we can conclude nothing from this, which means that in this lane there was no mpt target in.
02:24
Coming to the lane, we explain the presence of three sets of band.
02:30
At the very top below the valve here, this region, and the faint band, this region, here is the faint band, and the fuzzy band below 100 base here, this band.
02:48
We have to explain all of them.
02:51
The top band in lane 3 shows the non -specific amplification of these...