While performing PCR, the step of annealing promotes __________ and is performed at _____ Synthesis of new DNA strands by DNA polymerase; In the range of 55-65 degrees Celsius (depending on Tm of primers) Denaturation of template; 95 degrees Celsius Binding of primers; In the range of 55-65 degrees Celsius (depending on Tm of primers) Synthesis of new DNA strands by DNA polymerase; 72 degrees Celsius
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Step 1: The annealing step in PCR promotes the binding of primers to the single stranded DNA template. Show more…
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What happens during the annealing step in PCR? - The two DNA strands separate at high temperatures. - The DNA and master mix are added together. - Primers bind to the newly separated DNA strand. - New DNA is made.
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