Why do proteins enter the gel during gel electrophoresis? Explain why proteins separate based on size in the SDS polyacrylamide gel. ionic detergent SDS denatures + binds to pro- them uniformly negatively charged, when
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Proteins are loaded onto the gel and migrate through the matrix towards the positive electrode. The gel matrix acts as a molecular sieve, with smaller molecules moving more easily through the pores than larger molecules. In SDS-PAGE (sodium dodecyl sulfate Show more…
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