Question

1. Why was a saline (0.9% NaCl) solution instead of distilled water used to remove cheek cells during a goggle rinse? 2. Why was instagene, which contains Chelex (binds divalent ions) added to the tubes prior to boiling the buccal cells? (Hint: Eukaryotic cells contain many heavy metals such as Mg2+, Mn2+, Ca2+, etc.). 3. Cells were boiled to lyse cells. Why does this preparation not destroy the integrity of DNA for PCR use? Is DNA denatured? If so, what bond(s) is broken? 4. Why are the tubes centrifuged a final time before the pure DNA can be removed and stored into clean tubes?

          1. Why was a saline (0.9% NaCl) solution instead of distilled water used to remove cheek cells during a goggle rinse?
2. Why was instagene, which contains Chelex (binds divalent ions) added to the tubes prior to boiling the buccal cells? (Hint: Eukaryotic cells contain many heavy metals such as Mg2+, Mn2+, Ca2+, etc.).
3. Cells were boiled to lyse cells. Why does this preparation not destroy the integrity of DNA for PCR use? Is DNA denatured? If so, what bond(s) is broken?
4. Why are the tubes centrifuged a final time before the pure DNA can be removed and stored into clean tubes?

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1. Why was a saline (0.9% NaCl) solution instead of distilled water used to remove cheek cells during a goggle rinse?
2. Why was instagene, which contains Chelex (binds divalent ions) added to the tubes prior to boiling the buccal cells? (Hint: Eukaryotic cells contain many heavy metals such as Mg2+, Mn2+, Ca2+, etc.).
3. Cells were boiled to lyse cells. Why does this preparation not destroy the integrity of DNA for PCR use? Is DNA denatured? If so, what bond(s) is broken?
4. Why are the tubes centrifuged a final time before the pure DNA can be removed and stored into clean tubes?

Added by Victoria F.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

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Solved by Expert Adi S

06/30/2023

Step 1

A saline solution (0.9\% NaCl) was used instead of distilled water to remove cheek cells during the gargle rinse because the saline solution is isotonic to the cells. This prevents the cells from undergoing osmotic shock, which could cause them to burst (lyse) if Show more…

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Why was a saline (0.9% NaCl) solution instead of distilled water used to remove cheek cells during a gargle rinse? Why was Instagene, which contains Chelex (binds divalent ions), added to the tubes prior to boiling the buccal cells? (Hint: Eukaryotic cells contain many heavy metals such as Mg2+, Mn2+, Ca2+, etc.) Cells were boiled to lyse cells. Why does this preparation not destroy the integrity of DNA for PCR use? Is DNA denatured? If so, what bond(s) is broken? Why are the tubes centrifuged a final time before the pure DNA can be removed and stored into clean tubes?

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