You are told to create a standard curve using the Bovine Serum Albumin (BSA) that is used in a Bradford Assay, which is good for measuring protein concentrations in the range between 0.5 and 1,000 µg/mL. There is no information on the suspected concentration of the unknown. For this practice worksheet, assume your BSA stock solution for Part 2 has a concentration of 0.2 mg/mL. Decide how many standards you want to make, and their concentrations. You can choose as many standards as you deem necessary to create an accurate and reliable standard curve. Then complete the table below to determine what dilutions of your stock BSA solution in water you will need in order to create your standards. You will need to calculate how much water and BSA stock solution is required to obtain a final volume of 300 µL.
Concentration of Standard BSA (mg/mL)
Volume of Water (µL)
Volume of BSA (µL)
Total Volume (µL)
0 (blank)
300
0
300
0.5
225
75
300
1
150
150
300