You believe that dendritic cells cluster together in a particular region of the lymph node in the days after an infection. What assay could you use to test your hypothesis? A) Flow Cytometry B) Fluorescent Microscopy C) Single Cell RNA sequencing D) An ELISA
Added by April S.
Step 1
** Show more…
Show all steps
Your feedback will help us improve your experience
Adi S and 101 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
1. I want to analyze if T cells derived from SARS-CoV-2 immunized mice can be properly activated or not. Which of the following assays can't be used to study T cell activation? A. Visualization of TCR or BCR crosslinking using immunofluorescence. B. Measure lymphocyte proliferation using CFSE staining and flow cytometry. C. Cytokine secretion using ELISA. D. H3 thymidine incorporation into RNA to measure mitosis. E. Cytokine production by lymphocytes using intracellular staining and flow cytometry. 2. I am considering using a mitogen to unspecifically stimulate the T cells. Which of the statements is incorrect regarding mitogens from the list? A. Mitogens do not elicit any immune responses. B. A mitogen is a chemical substance that encourages a cell to commence cell division, triggering mitosis. C. Mitogens are only found in mice. D. Mitogens are agents that nonspecifically activate lymphocytes. E. Mitogens act as small peptides that fit in most TCR and activate T cells. F. Mitogens bind and cross-link components of the TCR and BCR, inducing intracellular signaling. G. Mitogens bind CD3 to activate cell proliferation. 3. Which antigens or molecules will not stimulate the proliferation of T cells derived from COVID-19 patients? A. CD3 B. CD67 C. IL-2 D. Spike-S/RBD E. IL-45 F. CD4 G. PHA
Adi S.
Which technique could you use if you wanted to compare all of the RNAs produced in a tumor vs a normal tissue in a single experiment? A. FISH (fluorescent in situ hybridization) B. immunocytochemistry C. cDNA microarray D. teratoma assay E. immunohistochemistry
Joy C.
QUESTION 41 ELISA Can measure protein abundance Requires antibodies Can be executed using a sandwich protocol Typically uses a multiwell plate All of the above QUESTION 42 You are working with a human cell type in culture and you need to know if it is regarded as a cell line or a cell strain. What parameter could be analyzed in this regard to distinguish the difference. The size difference between the two cell types The ability of one but not the other to enter cell senescence and then apoptosis after a few dozen doublings. The ability of one but not the other to grow in media containing serum The ability of one but not the other to go through the cell cycle abnormally fast The ability of one but not the other to adhere to cell culture microbeads QUESTION 43 Teloyears is a company that can tell you how long your telomeres are. Where would you find telomeres? Tips of chromosomes Cell membrane exoplasmic (outside) surface Mitochondrial DNA Expressed exclusively in cancer stem cell cytoplasm Circulating as a protein in your blood QUESTION 44 You are working with tissue sections from a tumor and view an interesting cell within this tumor that you think is a cancer stem cell. You would like to remove this cell so that you can subsequently analyze it using single cell analysis. Which microscope would be best in this regard? Two photon microscopy Spinning disk confocal microscopy Laser capture microdissection microscopy Transmission electron microscopy High voltage electron microscopy QUESTION 45 The insulin receptor was purified for the first time several decades ago relying on the ability of insulin to bind to the receptor. Which technique was used in this regard? Gel filtration SELDI-TOF DEAE ion exchange chromatography Protein A immunoprecipitation Affinity chromatography QUESTION 46 The elution of proteins from ion exchange chromatography beads is the last critical step for protein separation and isolation. What is typically used for this purpose? EGTA Liberase Triton-X 100 Sodium chloride Protein A QUESTION 47 "Pigs have been considered as possible candidates to partially address the organ transplant supply demand curve which is out of balance. While transplanting a pig organ (xenotransplantation) is not allowed in the US and other countries, pigs may be good candidates for future organ transplantation. Why is this the case?" Their organs are of similar size to humans Their tissues when implanted result in a very minimal immune response They contain no viruses in their genome making them safe for transplant They contain unique B cells which when injected with a transplanted pig organ prevents rejection All of the above QUESTION 48 "You are working in a laboratory and processing a tissue using formaldehyde. At the end of the processing procedure you stain the tissue section with hematoxylin and eosin that appear purple and pink, respectively, in the microscope. Which microscope is best designed to viewing this tissue?" Spinning-disk confocal microscope Fluorescence microscope Phase microscope Bright field/compound light microscope Atomic force microscope QUESTION 49 Cytochrome p450 is commonly lost over time in primary human hepatocytes. What does cytochrome p450 do? It repairs telomeres It detoxifies substances and generates water soluble products that are secreted It ensures that the mitochondria in aging cells stay active It adds phosphates and carbohydrate groups to newly synthesized proteins The function of this enzyme is not yet known QUESTION 50 Bispecific antibodies have advantages or limitations over native monoclonal antibodies given that unlike native monoclonal antibodies bispecific antibodies Can bring three different cell types together in close proximity that in select cases can result in apoptosis of a targeted cell Can be used with gold particles for labeling proteins Can be used in ELISA assays whereas traditional monoclonal antibodies can't be used for this purpose Can be visualized using phase microscopy unlike traditional monoclonal antibodies All of the above
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD