You conduct a PCR reaction with two PCR machines in your lab. At the end of the reactions you realize that both amplifications have failed. b) For the second machine, you find that the machine does not increase temperature beyond 70 degrees Celsius. Which part of the PCR cycle was hampered? What would you see after gel electrophoresis? Choose from the following: A) There would be no bands on the gel because the temperature at step 1 was too high for the DNA strands to separate B) There would be no bands on the gel because the temperature at step 1 was too low for the DNA strands to separate C) There would be no bands on the gel because the temperature at step 2 was too high for the primers to anneal D) There would be extra bands on the gel because the temperature at step 2 was too low for the bands to bind specifically to only one gene E) There would be no bands on the gel because the temperature at step 2 was too low for the primers to anneal
Added by Francisco Jose H.
Step 1
In this case, the machine does not increase the temperature beyond 70°C, so the DNA strands would not separate properly. Step 2 of PCR is annealing, where primers bind to the target DNA sequence. This step usually occurs at a temperature between 50-65°C. Since Show more…
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