Regarding PCR The last procedure in the laboratory was to run the samples in an agarose gel electrophoresis. If you detected DNA bands in some of the samples, what will be the next step of the project? A) Throw all samples away. B) Use those samples for a new nested PCR. C) Sequence the samples that showed bands in the gel. D) Sequence the samples that did not have bands on the gel. E) Use the samples for a new ligation reaction. You need to design primers to isolate gene X from a plant species using PCR and genomic DNA as a template. You have the sequence of the homologous gene X in other plant species. Which regions of gene X will you use to target the primers? A) Introns B) Exons C) 3' UTR D) 5' UTR E) PolyA
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This is because the presence of bands indicates that the PCR was successful in amplifying the target DNA sequence. Sequencing these samples will allow you to confirm the identity of the amplified DNA and ensure that it is the correct target sequence. Second, when Show more…
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A thorough understanding of the process of PCR will help in the analysis of your DNA gel. The figure below represents a segment of double-stranded DNA with 100 base pair segments denoted by each letter. The primers are indicated by the arrows for both the sense and anti-sense DNA strands. In the following questions, a "copy" of DNA refers to a double-stranded piece of DNA. A. Draw the first three cycles of PCR indicating the intermediate products labeled with letter designations, directionality (5' → 3'), and the size of the desired target double-stranded DNA product. Include the temperatures and their significance for each step in the PCR reaction for the first cycle. (9 points, 3 pts per cycle) B. For each cycle, indicate how many copies of target double stranded-DNA and "intermediate DNA" (the DNA includes target DNA region plus a bit of the flanking DNA and/or the original DNA strand) you would have after each cycle assuming you began with one double strand DNA template. (6 points, 2 pts per cycle)
Farhan A.
You conduct a PCR reaction with two PCR machines in your lab. At the end of the reactions you realize that both amplifications have failed. b) For the second machine, you find that the machine does not increase temperature beyond 70 degrees Celsius. Which part of the PCR cycle was hampered? What would you see after gel electrophoresis? Choose from the following: A) There would be no bands on the gel because the temperature at step 1 was too high for the DNA strands to separate B) There would be no bands on the gel because the temperature at step 1 was too low for the DNA strands to separate C) There would be no bands on the gel because the temperature at step 2 was too high for the primers to anneal D) There would be extra bands on the gel because the temperature at step 2 was too low for the bands to bind specifically to only one gene E) There would be no bands on the gel because the temperature at step 2 was too low for the primers to anneal
Adi S.
Let's suppose you want to clone a gene that has never been analyzed before by DNA sequencing. Which of the following statements do you agree with the most? a. Do $\mathrm{PCR}$ to clone the gene because it is much faster. b. Do PCR to clone the gene because it is very specific and gives a high yield. c. You can't do PCR because you can't make forward and reverse primers. d. Do cloning by insertion into a vector because it will give you a higher yield. e. Do cloning by insertion into a vector because it is easier than $\mathrm{PCR}$.
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