You have isolated a protein that you think is encoded within a small region of DNA. In this region, there are 3 open reading frames (ORFs). Open reading frames are DNA sequences that are likely genes (have typical promoters, start and stop codons). ORF #1 has an open reading frame of 1500 nucleotides (AUG to stop codon). ORF #2 has an open reading frame of 600 nucleotides. ORF #3 has an open reading frame of 900 nucleotides. The protein you isolated is 33 kDa in size. If the average amino acid mass is 110 Da, then which of the above 3 ORFs most likely codes for this protein? a) ORF #1 b) ORF #2 c) ORF #3 d) None of the ORFs are large enough to encode this protein
Added by Emily J.
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1 kDa = 1000 Da, so a 33 kDa protein is 33,000 Da. Show more…
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You have isolated a protein that you think is encoded within a small region of DNA. In this region there are 3 open reading frames (ORFs). Open reading frames are DNA sequences that are likely genes (have typical promoters, start and stop codons). ORF #1 has an open reading frame of 1500 nucleotides (AUG to stop codon) ORF #2 has an open reading frame of 600 nucleotides ORF #3 has an open reading frame of 900 nucleotides The protein you isolated is 33kDa in size. If the average amino acid mass is 110 Da, then which of the above 3 ORFs most likely codes for this protein?
Keemin L.
Question: In a gene, an Open Reading Frame (ORF) is the protein coding sequence that begins with a start codon and ends with a stop codon. Please examine the following two DNA sequences and predict the amino acid sequences of the possible proteins translated from all possible ORFs (not reading frames!) of the possible mRNAs transcribed from the double-stranded DNA. <DNA_A> 5’-GAGGCGATGCGCGATTGGAGCAAGGATCGATATCAGTGACGAGCTA-3’ 3’-CTCCGCTACGCGCTAACCTCGTTCCTAGCTATAGTCACTGCTCGAT-5’ <DNA_B> 5’-GCGATCGTAGCTAGCGGCGACGACTAGCTAGCTGGATCGACATAGCA-3’ 3’-CGCTAGCATCGATCGCCGCTGCTGATCGATCGACCTAGCTGTATCGT-5’
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Below are several DNA sequences that are mutated compared with the wild-type sequence: $3^{\prime}-T$ A $C$ T $G A$ C T G A C G A T C-5'. Envision that each is a section of a DNA molecule that has separated in preparation for transcription, so you are only seeing the template strand. Construct the complementary DNA sequences (indicating $5^{\prime}$ and 3 ' ends) for each mutated DNA sequence, then transcribe (indicating 5 ' and 3 ' ends) the template strands, and translate the mRNA molecules using the genetic code, recording the resulting amino acid sequence (indicating the $\mathrm{N}$ and C termini). What type of mutation is each?
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