You have isolated a protein that you think is encoded within a small region of DNA. In this region there are 3 open reading frames (ORFs). Open reading frames are DNA sequences that are likely genes (have typical promoters, start and stop codons). ORF #1 has an open reading frame of 1500 nucleotides (AUG to stop codon) ORF #2 has an open reading frame of 600 nucleotides ORF #3 has an open reading frame of 900 nucleotides The protein you isolated is 33kDa in size. If the average amino acid mass is 110 Da, then which of the above 3 ORFs most likely codes for this protein?
Added by Meghan O.
Step 1
1 kDa equals 1000 Da, so a 33 kDa protein is 33,000 Da. Show more…
Show all steps
Your feedback will help us improve your experience
Keemin Lee and 74 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
You have isolated a protein that you think is encoded within a small region of DNA. In this region, there are 3 open reading frames (ORFs). Open reading frames are DNA sequences that are likely genes (have typical promoters, start and stop codons). ORF #1 has an open reading frame of 1500 nucleotides (AUG to stop codon). ORF #2 has an open reading frame of 600 nucleotides. ORF #3 has an open reading frame of 900 nucleotides. The protein you isolated is 33 kDa in size. If the average amino acid mass is 110 Da, then which of the above 3 ORFs most likely codes for this protein?
Sana M.
You have isolated a protein that you think is encoded within a small region of DNA. In this region, there are 3 open reading frames (ORFs). Open reading frames are DNA sequences that are likely genes (have typical promoters, start and stop codons). ORF #1 has an open reading frame of 1500 nucleotides (AUG to stop codon). ORF #2 has an open reading frame of 600 nucleotides. ORF #3 has an open reading frame of 900 nucleotides. The protein you isolated is 33 kDa in size. If the average amino acid mass is 110 Da, then which of the above 3 ORFs most likely codes for this protein? a) ORF #1 b) ORF #2 c) ORF #3 d) None of the ORFs are large enough to encode this protein
Josee P.
Sri K.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
Watch the video solution with this free unlock.
EMAIL
PASSWORD