You have just completed a microarray analysis to determine which genes are expressed downstream of a receptor X. You divide a population of cells in two. You add ligand to half the population and add nothing to the other half (your control). After 24 hours, you isolate the mRNA from the two cell populations and create cDNA from both sets of mRNA. You tag the cDNA from the control cells with a green fluorescent probe and tag the cDNA from the ligand-treated cells with a red fluorescent probe. You add both sets of labeled cDNA to a microarray chip & analyze the results. What can you conclude about a gene whose spot on the chip is red? a. Receptor X signaling results in increased expression of the gene b. Receptor X signaling results in decreased expression of the gene c. Receptor X signaling has no effect on expression of the gene d. The gene is not expressed in control or ligand-treated cells
Added by Craig R.
Step 1
The experiment involves dividing a population of cells into two groups: one treated with ligand and the other untreated (control). Show more…
Show all steps
Your feedback will help us improve your experience
Nayan G and 61 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Jenny W.
You have just completed a microarray analysis to determine which genes are expressed downstream of receptor X. You divide a population of cells in two. You add ligand to half the population and add nothing to the other half (your control). After 24 hours, you isolate the mRNA from the two cell populations and create cDNA from both sets of mRNA. You tag the cDNA from the control cells with a green fluorescent probe and tag the cDNA from the ligand-treated cells with a red fluorescent probe. You add both sets of labeled cDNA to a microarray chip and analyze the results. What can you conclude about a gene whose spot on the chip is red? a. Receptor X signaling results in increased expression of the gene b. Receptor X signaling results in decreased expression of the gene c. Receptor X signaling has no effect on expression of the gene d. The gene is not expressed in control or ligand-treated cells
The following microarray gene expression data are shown for four human genes. Two samples were hybridized to the microarray in this experiment. One sample was cDNA generated from a cancerous melanocyte in the skin tissue. The other sample was cDNA generated from normal skin tissue. Gene: 1 2 3 4 means hybridization to cancerous skin cell cDNA only mean hybridization to normal skin cell cDNA only means hybridization to both cDNA types means hybridization to neither cell's cDNA Which of these genes is over-expressed in a cancerous skin cell and what is a possible function for this gene, based on the microarray data? Gene 3; this gene is likely an oncogene involved in stimulating cell growth, so it is turned on in the cancer cell line that is growing rapidly Gene 4; this gene is likely involved in maintenance of telomerase function, so it is not expressed in either normal or cancerous cell Gene 1; this gene is likely involved in neurotransmitter release at the synapse, so it is switched on in the cancer cell line sending signals to grow rapidly Gene 2; this gene is likely a tumor suppressor involved in inhibiting cell growth, so it is over-expressed in the cancer and normal cell
Supreeta N.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Watch the video solution with this free unlock.
EMAIL
PASSWORD