Question

3. You have the following PCR product. The internal restriction enzymes are indicated and the distance of each from the left side in basepairs are shown below. 0 EcoRI Smal EcoRI 65 95 145 250 On the agarose gel image below, draw and label the sizes of bands that you would expect to find for the following lanes: Uncut DNA, DNA cut with EcoRI alone, DNA cut with Smal alone, DNA cut with both EcoRI and Smal.

          3. You have the following PCR product. The internal restriction enzymes are indicated and the distance of each from the left side in basepairs are shown below.
0
EcoRI
Smal
EcoRI
65
95
145
250
On the agarose gel image below, draw and label the sizes of bands that you would expect to find for the following lanes: Uncut DNA, DNA cut with EcoRI alone, DNA cut with Smal alone, DNA cut with both EcoRI and Smal.

3. You have the following PCR product. The internal restriction enzymes are indicated and the distance of each from the left side in basepairs are shown below.
0
EcoRI
Smal
EcoRI
65
95
145
250
On the agarose gel image below, draw and label the sizes of bands that you would expect to find for the following lanes: Uncut DNA, DNA cut with EcoRI alone, DNA cut with Smal alone, DNA cut with both EcoRI and Smal.

Added by Denise K.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

Instant Answer

Solved by Expert Jenny Wu

05/19/2023

Step 1

Step 1: For the first PCR product with internal restriction enzymes EcoRI and SmaI, we need to determine the sizes of the fragments that would be produced after cutting with each enzyme or both enzymes. Show more…

Show all steps

Thanks for your feedback!

You have the following PCR product. The internal restriction enzymes are indicated and the distance of each from the left side in base pairs are shown below. 1 50 On the agarose gel image below, draw and label the sizes of bands that you would expect to find for the following lanes: Uncut DNA, DNA cut with EcoRI alone, DNA cut with SmaI alone, DNA cut with both EcoRI and SmaI. 3. You have the following PCR product. The internal restriction enzymes are indicated and the distance of each from the left side in base pairs are shown below. EcoRI SmaI EcoRI 0 65 95 145 250 On the agarose gel image below, draw and label the sizes of bands that you would expect to find for the following lanes: Uncut DNA, DNA cut with EcoRI alone, DNA cut with SmaI alone, DNA cut with both EcoRI and SmaI.