You isolate mitochondria from wildtype (WT) and mutant (M) animals. You measure ATP production from either NADH or FADH_(2) in these mitochondria. Based on these data, which components of the ETC could, MOST LIKELY, be defective in the mutants (select all that apply). Complex IV Complex II Complex I Complex III
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Step 1
- Complex I (NADH dehydrogenase) accepts electrons from NADH. - Complex II (succinate dehydrogenase) accepts electrons from FADH2. - Complex III (cytochrome bc1 complex) transfers electrons from both Complex I and II to Complex IV. - Complex IV (cytochrome c Show more…
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A 19-year-old male is being evaluated for muscle weakness. Evaluation of a muscle biopsy revealed normal rates of succinate oxidation but very low rates of pyruvate oxidation. Further testing determined normal levels of enzymatic activity in both malate dehydrogenase and pyruvate dehydrogenase. Based on this data, the patient may have a mutation in a gene encoding for which mitochondrial protein? Complex I (NADH Q-oxidoreductase) Complex II (Succinate Q-reductase) Complex III (Q-cyt c oxidoreductase) Complex IV (Cytochrome oxidase) Complex V (ATP synthase)
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