Question

You would like to produce large amounts of a protein encoded by the gene wmu1 in E. coli using a specific plasmid. a). Draw an example of a plasmid that may be used in the expression of this gene with all required elements for expression in E. coli. b). What element(s) on the plasmid should you optimize in order to improve transcription of gene wmu1? c). What element(s) on the plasmid should you optimize in order to increase the replication of the plasmid in E. coli?

Name: you would like to produce large amounts of a protein encoded by the gene wmu1 in e coli using a specific plasmid a draw an example of a plasmid that may be used in the expression of this gen 27285
Uploaded: 2022-03-25T22:46:55-08:00
Duration: 1 min 14 s
Channel: Keemin Lee
Description: you would like to produce large amounts of a protein encoded by the gene wmu1 in e coli using a specific plasmid a draw an example of a plasmid that may be used in the expression of this gen 27285

          You would like to produce large amounts of a protein encoded by the gene wmu1 in E. coli using a specific plasmid.
a). Draw an example of a plasmid that may be used in the expression of this gene with all required elements for expression in E. coli.
b). What element(s) on the plasmid should you optimize in order to improve transcription of gene wmu1?
c). What element(s) on the plasmid should you optimize in order to increase the replication of the plasmid in E. coli?

Added by Josefina R.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

Instant Answer

Solved by Expert Keemin Lee

03/25/2022

Step 1

Step 1:** Draw a plasmid with the following elements: - Blue region: Gene wmu1 - Red region: Antibiotic resistance gene - Promoter region for gene expression ** Show more…

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You would like to produce large amounts of a protein encoded by the gene wmu1 in E. coli using a specific plasmid. a). Draw an example of a plasmid that may be used in the expression of this gene with all required elements for expression in E. coli. b). What element(s) on the plasmid should you optimize in order to improve transcription of gene wmu1? c). What element(s) on the plasmid should you optimize in order to increase the replication of the plasmid in E. coli?