00:01
Is making a 1 to 10 dilution of the stock.
00:10
So you want to make accurately one milliliter final volume.
00:17
So what you have is that you have a 1x full -dye solution and you have a disposable micro tubes that hold two milliliter in total and you have 100 to 1000 microliter micro pipette.
00:34
So what we know is that we want to know, we have the final volume, this is one milliliter.
00:41
And we want to make a 1 to 10 dilution.
00:43
This means that the dilution factor is 10.
00:56
So we know the formula that the initial volume divided by the final volume equals 1 to 10.
01:11
10 is the dilution factor.
01:13
So we want to figure out initial volume.
01:15
We know the final volume is one mil.
01:17
So initial volume, we adjust this formula equals 1 10th times the final volume.
01:28
And we know that the final volume is going to be one mil.
01:31
So we plug that in, 1 10th times one milliliter, which is 0 .1 milliliter.
01:39
So now we know that we have micro pipettes that are 100 to 1000 microliter...