Question
An enzyme has the following pH dependence: At what pH do you think this enzyme works best?
Step 1
If the pH is lower than 7 or higher than 11, the enzyme becomes denatured and loses its structure, which disrupts its function. Show more…
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The activity of many enzymes is pH dependent. For example, the optimum activity of two common digestive enzymes trypsin and pepsin occur at $\mathrm{pH} 6.5$ and $\mathrm{pH} 1.5$ respectively. Using the data in Appendix 5 as a guide, which buffer would you choose for each enzyme to maximize their activity?
What is meant by the optimum pH for an enzyme? (20.3)
Enzymes and Vitamins
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The catalytic efficiency of an enzyme, called its activity, indicates the rate at which it catalyzes the reaction. Most enzymes have optimum activity over a relatively narrow pH range, which is related to the pH of the local cellular fluid. The pH profiles of three digestive enzymes are shown.
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