Question
Compare and contrast the principles of autoradiography and phosphorimaging. Which method provides more quantitative information?
Step 1
When the sample is exposed to a photographic film or a detector, the emitted radiation from the isotopes exposes the film, creating an image that reflects the location and intensity of the radioactivity. Show more…
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Autoradiography depends on particles emitted from radioactive atoms striking a photographic emulsion that lies on top of the tissue section. When the emulsion is developed, the site where the particle struck the emulsion appears as a silver grain, as in Figure 8.3 a. How do you think the thickness of the section might affect the resolution of the technique, that is, the ability to locate the precise site in the cell where radioactivity is incorporated?
Autoradiography depends on particles emitted from radioactive atoms striking a photographic emulsion that lies on top of the tissue section. When the emulsion is developed, the site where the particle struck the emulsion appears as a silver grain, as in Figure $8.3 a .$ How do you think the thickness of the section might affect the resolution of the technique, that is, the ability to locate the precise site in the cell where radioactivity is incorporated?
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