00:01
For this question, we're looking at how to do a direct count on a bacterial sample.
00:06
So the reason we need to do a bacterial direct count is because we're going to be culturing a tube, typically with a broth inside of it, and we're going to need to measure the amount of bacteria inside of it by direct numeration.
00:22
However, we can't just guess at it.
00:24
It can be difficult to relate it to optical density or the amount of light scale.
00:30
Scatter through the bra.
00:31
So we often do it through a direct counting by hand.
00:36
So to do this, we're going to use a microscope.
00:42
And what we do is we're going to relate an amount of volume that we use to look at with the microscope, and we are going to do that count, to see how many bacteria we have.
00:54
From there, we can relate the volume we used for the microscope and relate it to the volume of the tube.
01:00
And we should get a direct count that relates to the volume here, such as, say, m1 v1 equals m2 v2, where your m here would be the count you get for each of them...