00:01
Okay, this is a long question, but we can break it down into parts and we don't have to get overwhelmed by it.
00:08
The first part of this question is talking about a mutation in dna that generates a uga stop codon in the middle of an mrna coding for a particular protein.
00:21
So what we have down here is let's say, and we'll highlight this in green, this is the mrna that we need to be changed.
00:30
Translated and this is the mutation right here, this uga, that is causing the stop codon to enter erroneously.
00:42
We don't want to stop this synthesis, but we are because of the sequence uga and it's being paired to a trna that does not have an amino acid associated with it.
00:55
So it's stopping that translation prematurely.
00:59
A second mutation, and the second part of this question, is talking about a mutation that leads to a single nucleotide change in the t -r -n -a that allows for the correct translation of the protein.
01:12
So this is where we're going to come across a new term, excuse me, and that's going to be snip, and that is a single nucleotide point mutation.
01:23
Actually, i guess the abbreviation is just snp.
01:26
P, but i have always heard it pronounced snip.
01:30
And here we're going from, now actually, let me back up again.
01:35
We're talking about a single nucleotide point mutation in the t -r -n -a...