Mutation # | DNA Sequence change | mRNA codon change (original > mutant) | Amino acid change (original > mutant) | Type of mutation (silent, missense, nonsense)
--- | --- | --- | --- | ---
1 | Coding ATA -> ATC, Template TAT -> TAG | AUA > AUC | Ile > Xxx |
2 | Coding TAT -> TAA, Template ATA -> ATT | UAU > NNN | Xxx > Xxx |
3 | Coding AGT -> AGG, Template TCA -> TCC | NNN > NNN | Xxx > Xxx |
D. Imagine a ‘G’ were inserted at the beginning of the original coding DNA sequence from part B as indicated here: 5'-GACG ATA TAT AGT GCT-3'
1. Write out the resulting mRNA and amino acid sequence.
New mRNA = 5'- GAC GAU AUA UAG UGC U-3'
New amino acid sequence = Asp Asp
2. What kind of mutation has occurred? Highlight
3. Do you think this mutation will affect the function of the protein? Why or why not? Highlight
3. Unrepaired DNA damage can lead to mutations.
a. DNA damage can be caused by mutagens in the environment. Give an example of a mutagen and the kind of DNA damage it causes.
Example Mutagen | Type of damage caused by the mutagen
b. A type of DNA damage called ‘depurination’ is a common, spontaneous form of DNA damage in which the nitrogenous base of a purine nucleotide is lost. How does the loss of the base affect the genetic information? Highlight