Another result you did not expect! But that is science! You then decide to perform another experiment.
Experiment 2: You have noticed your labgrage is getting a little messy and some moths have started laying eggs in the corner of the room. While Dr. Romo has not mentioned this yet, you know (because you spend your free time reading mycology research papers) that people use wax moth (Galleria mellonella) larvae as an infection model because they get sick from fungal infections and die. This is a good way to measure how virulent a fungus is! You pick up a few of these moths from your labrage and give them a buffer (PBS) (black squares), C. albicans yeasts (black circles), OR C. albicans yeasts that were treated with EVs before you infected the larvae (black diamonds). You then follow them for 8 days and measure percent survival for each group.
Notes: PBS is just a buffer, basically saline. This is your negative control. It should not have any effect on the larvae. The 90028 is just a strain number. Pay no attention to it.
Question 2d. What happened with each of the 3 groups? What was the effect of the EVs regarding virulence of C. albicans in this experiment? (5 points)
Question 2e. What is your overall interpretation of the effect of EVs on C. albicans biology? Hypothesize why the fungus would use EVs in this way in the human host? (5 points)
