Question

Bradford assay: 1) Add 4 ml of water to 1 ml of Bradford reagent. 2) Make standards of BSA: 1 mg/ml, 0.75 mg/ml, 0.5 mg/ml, 0.25 mg/ml, and 0.125 mg/ml. 3) Fill wells from columns 1-7 in rows A-C with 200 ul of diluted Bradford reagent. 5) Add 10 ul of H2O, standard, or your sample to 200 ul of Bradford reagent as shown below. Mix well while pipetting! BSA standard your sample H2O H2O H2O mg/ml 0.75 mg/ml 0.5 mg/ml 0.25 mg/ml 0.125 mg/ml 1 mg/ml 0.75 mg/ml 0.5 mg/ml 0.25 mg/ml 0.125 mg/ml mg/ml 0.75 mg/ml 0.5 mg/ml 0.25 mg/ml 0.125 mg/ml 6) Wait 5 minutes and measure the absorbance at 595 nm using Magellan Teacan plate reader. Export the read data to Microsoft Excel. 8) Calculate the standard curve.

          Bradford assay:
1) Add 4 ml of water to 1 ml of Bradford reagent.
2) Make standards of BSA: 1 mg/ml, 0.75 mg/ml, 0.5 mg/ml, 0.25 mg/ml, and 0.125 mg/ml.
3) Fill wells from columns 1-7 in rows A-C with 200 ul of diluted Bradford reagent.
5) Add 10 ul of H2O, standard, or your sample to 200 ul of Bradford reagent as shown below. Mix well while pipetting!
BSA standard
your sample
H2O H2O H2O
mg/ml 0.75 mg/ml 0.5 mg/ml 0.25 mg/ml 0.125 mg/ml 1 mg/ml 0.75 mg/ml 0.5 mg/ml 0.25 mg/ml 0.125 mg/ml mg/ml 0.75 mg/ml 0.5 mg/ml 0.25 mg/ml 0.125 mg/ml
6) Wait 5 minutes and measure the absorbance at 595 nm using Magellan Teacan plate reader.
Export the read data to Microsoft Excel.
8) Calculate the standard curve.

Added by Gabriel N.

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