3. Design an experiment using Beadle and Tatum's experimental design, with growth or no growth as an outcome, to test your hypothesis whether one (or both) mutation(s) you found in the ornithine carbamoyltransferase gene causes this strain's inability to synthesize arginine from minimal medium. Draw your experimental design on your poster. Don't forget your controls. Show the results you'd expect if your hypothesis is correct. Use the images below as a guide. Assumption: You have the ability to manipulate the DNA sequence within that yeast strain any way you want and can replace the DNA sequence found in any gene in the yeast cell with another sequence. Gene A -> Enzyme A Gene B -> Enzyme B Gene C -> Enzyme C Precursor -> Ornithine -> Citrulline -> Arginine Growth Media | Wild-type | New Mutant | Experiment | Experiment | Experiment | Experiment | Experiment | Experiment Minimal | Growth | NO Minimal + Ornithine | Growth | ? Minimal + Citrulline | Growth | ? Minimal + Arginine | Growth | Growth
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Design an experiment using Beadle and Tatum's experimental design, with growth or no growth as an outcome, to test your hypothesis whether one (or both) mutation(s) you found in the ornithine carbamoyltransferase gene causes this strain's inability to synthesize arginine from minimal medium. Draw your experimental design on your poster. Don't forget your controls. Show the results you'd expect if your hypothesis is correct. Use the images below as a guide. Assumption: You have the ability to manipulate the DNA sequence within that yeast strain any way you want and can replace the DNA sequence found in any gene in the yeast cell with another sequence.
Sri K.
Imagine you do a version of the Beadle-Tatum experiment in which you attempt to test the one-gene, one-enzyme hypothesis, but using the pathway of synthesis of amino acid Q from X (below). The reactions, in order, are catalyzed by enzymes E1-E5. X is always present in cells, and cells need Q to grow. X -> Y -> Z -> P -> D -> Q. You identify an auxotroph that doesn't grow in normal medium, but does grow in medium supplemented with Q, indicating that it has a defect in one or more enzymes in this pathway. This auxotroph is also able to grow if you supplement the medium with Y. Does this data support the one gene-one enzyme hypothesis? yes, because only one enzyme in the pathway is functional in the mutant strain no, because this doesn't rule out the possibility that more than one enzyme is disrupted by the mutation no, because this only tells you that Enzyme 1 is still functional yes, because only one enzyme is disrupted by the mutation
Jenny W.
Design an experiment to test your hypothesis. You have available to you: i. Plasmid DNA containing the GFP gene ii. Two DNA segments, each containing a different promoter sequence: pmtrA isolated from species A and pmtrB isolated from species B for the clrD gene. iii. Competent E. coli cells and all materials needed for bacterial transformation. Using the materials above and other appropriate laboratory techniques discussed in BIO 121, write a concise list of steps to design an experiment to determine the influence that each promoter has on gene expression. You do not need to include specific details about each laboratory technique you mention; however, be sure to include the appropriate controls. At the end of your list, make a prediction about the results of your experiment.
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