Prokaryotic termination: The consensus sequences are named -10 and -35 consensus sequence, and are located at Pribnow box and -35, respectively. Attachment of sigma factor to the RNA polymerase recognizes these consensus sequences.
Eukaryotic termination: The consensus sequences are named TATA Box and CAAT, and are located at -80 and -90, respectively. A multi-subunit protein complex binds to the consensus sequence.
Step 2 - Prokaryotic Initiation: How many polymerases are needed and what are they called?
Step 2 - Eukaryotic Initiation: How many polymerases are needed and what are they called?
Step 3 - Prokaryotic Elongation: Termination relies on the termination sequence containing inverted repeats and a string of adenines to create a series of U's that follow this structure, causing the polymerase to dissociate. Termination relies on the rho factor. This activates the polymerase to break H bonds between DNA and RNA.
Step 3 - Eukaryotic Elongation: Pre-translational modifications occur before termination. These include 3' cap and 5' cap. Termination then occurs via a special poly(A) tail that digests the leftover transcript until it has reached the intron splicing. This dissociates it from DNA. This is called the splicing model.