00:01
So in this experiment, we have a wild type competent bacteria that has basically been mixed with a plasmid to see if we can transform it via uptake of this dna of this plasma.
00:13
And this plasmid encodes for an ampicillin resistance gene.
00:16
So we have wild type or wild type with plasmid.
00:20
And then we have ampacillin or no ampacillin plates.
00:23
And then we see that, obviously, in the no ampacillin plates with bacteria, the blue, you see.
00:31
Growth all over the plate, we see a lawn of bacteria.
00:36
On the ampicillin plate, we see no bacteria.
00:40
Okay? that makes sense because the wild type bacteria is ampacillin sensitive.
00:44
So it would make sense that there would be no growth on the ampacillin plate.
00:49
On the back to the no ampacillin plate, if you grow the wild type with the plasma that transformed bacteria, we see growth everywhere.
00:58
Also makes sense, right? there's no antibiotic selection happening here, so it would make sense that, again, you see bacteria growing everywhere...