1. Given the DNA coding sequence 5'-...TAC AAA ATA CAG CGG...-3', which of these sequences represents a frameshift mutation? Assume that the start codon is localized upstream of this sequence.
A) 5'-...TAG AAA ATA CAG CGG...-3'
B) 5'-...TAC AAG ATA CAG CGG...-3'
C) 5'-...TAC AAA ATA CAC CGG...-3'
D) 5'-...TAC AAA ATA CAG AGG...-3'
E) 5'-...TAC AAA TAC AGC GGG...-3'
F) two of the above (A-E)
G) none of the above (A-E)
2. Given the DNA coding sequence 5'-...TAC AAA ATA CAG CGG...-3', which of these sequences represents a nonsense mutation? Assume that the start codon is localized upstream of this sequence.
A) 5'-...TAG AAA ATA CAG CGG...-3'
B) 5'-...TAC AAA TAC AGC GGG...-3'
C) 5'-...TAC AAG ATA CAG CGG...-3'
D) 5'-...TAC AAA ATA CAC CGG...-3'
E) 5'-...TAC AAA ATA CAG AGG...-3'
F) two of the above (A-E)
G) none of the above (A-E)
3. What would be the effect of a mutation in the lacI gene that prevents the repressor from binding to allolactose?
A) The lac Z, Y, and A genes would not be expressed.
B) The lac Z, Y, and A genes would be transcribed at basal level.
C) The lac Z, Y, and A genes would be repressed by lactose.
D) The lac Z, Y, and A genes would be transcribed at high level.
4a. ____ are mutations that have little or no effect on the fitness of the organism. (Do NOT put silent mutations)
4b. A ____ is a piece of RNA that can regulate gene expression when it interacts with certain metabolite(s).
4c. A/an ____ is a sequence of nucleotides that is the reversed complement of another sequence further downstream.
4d. A/an ____ is an effector that increases transcription by enabling an activator or disabling a repressor.