The typical cycle of a PCR reaction includes a period of time at 95 degrees, 55 degrees, and 72 degrees. Match the temperature to the event that happens at that temperature. A. 95 degrees - A. B. C. the DNA polymerase enzymes build new DNA at this temperature, starting from the 3' hydroxyl end of the annealed primers B. 55 degrees - A. B. C. the pre-synthesized primers find their complementary sites on the template and attach via hydrogen bonds at this temperature C. 72 degrees - A. B. C. the complementary strands of the DNA become separated at this temperature
Added by Susan F.
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Step 1: At 95 degrees Celsius, the complementary strands of the DNA become separated in a process called denaturation. Show more…
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In the PCR reaction, you need a three-step reaction cycle, which results in a chain reaction that produces an exponentially growing population of identical DNA molecules. Each step of a reaction cycle is usually performed at a different temperature. a) Name the three steps and state the approximate temperatures at which they take place. (6 marks) b) Using your knowledge of DNA structure, explain the design of these three temperature steps. (6 marks)
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