M8: ACTIVITY NO.1 Preparation of Culture Media and Inoculation of Microbes
OBJECTIVES: To be able to identify the different culture media and its usage.
To be able to describe process of preparing and sterilizing culture media.
To be able to describe the different inoculation methods.
NAMES:
SECTION:
1. Why is agar the most suitable used as culture medium of the microbial growth?
2. In the different types of culture media, what is the rationale of using:
a. Liquid medium such as nutrient broth over solid media like agar butt or agar plate.
b. Agar butt over agar slant
c. Agar slant over agar butt
d. Agar plate
3a. Describe the process of inoculating microbes
3b. Draw the inoculation technique done from a to c:
4. Describe: (Include how the process is done and what is purpose of these 2 techniques)
a. pour plate
b. spread plate
5. Explain:
a. Prior to its usage, why is sterilization of the culture media and equipment necessary? How is this process done?
b. In stabbing technique, why is path of withdrawal of the inoculating wire be the same path of inoculation?
c. During streaking technique, why is important not to cut the culture media?
d. After inoculation, why is necessary to incubate at 37°C for 48 hours?
e. Why are agar plates inverted during incubation?
6. Draw (Indicate the by numbers where it starts up to it ends)
Quadrant Streak
Radiant Streak
T- Streak
Continuous Streak