Molecular biologists can determine experimentally whether a region of DNA contains closed chromatin or open chromatin by assessing the sensitivity of the region to nucleosomes, RNA polymerase II, methyltransferase, DNAse, or histone deacetylase.
Added by Rachel M.
Step 1
Assess the sensitivity of the region to nucleosomes Show more…
Show all steps
Your feedback will help us improve your experience
Sri K and 51 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Eukaryotic gene regulation You isolate yeast chromatin and break it down to fragments with one or two nucleosomes. Using immunological (antibodies, no need to know any detail), you selectively isolate nucleosomes that have acetylated histone tails. You extract DNA from this purified fraction and sequence it. Comparing the sequence you obtain to the annotated genome sequence you expect to find the location of: A) active promoters B) crossing overs C) inactive promoters D) all nucleosomes E) transposons
Dominador T.
if you need to determine whether a sample of nucleic acids is RNA or DNA, which part of the nucleotides could you analyze to make the determination
Adi S.
By examining the compactness of a chromosome region (loose versus tightly wound), you can gain insight into whether the genes in that region are actively being used.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD