Question

Opening Prompt: As part of a study into the effects of paclitaxel on MDA-MB-231 cells you are asked to set up a small experiment where cells are treated with the drug prior to their isolation to create lysates. You have the following materials. In addition to deciding on the treatments and controls you will have to work out the dilutions required. After you have had time to come up with a plan we will discuss as a group. ? Isolated MDA-MB-231 cell suspension (5 mL total) at a concentration of 463, 000 cells / mL ? Paclitaxel dissolved in DMSO at a stock concentration of 10 mM ? DMSO ? Apoptosis inducing DNA damage agent Doxorubicin dissolved in DMSO at concentration of 10 mM (this drug is typically used at a final concentration of 5 µM to induce cell death) ? Coated 6 well tissue culture plates (1 - 2 mL volume of media is typically added to a well) ? Complete media

          Opening Prompt: As part of a study into the effects of paclitaxel on MDA-MB-231 cells you are asked to set up a small experiment where cells are treated with the drug prior to their isolation to create lysates. You have the following materials. In addition to deciding on the treatments and controls you will have to work out the dilutions required. After you have had time to come up with a plan we will discuss as a group.

? Isolated MDA-MB-231 cell suspension (5 mL total) at a concentration of 463, 000 cells / mL
? Paclitaxel dissolved in DMSO at a stock concentration of 10 mM
? DMSO
? Apoptosis inducing DNA damage agent Doxorubicin dissolved in DMSO at concentration of 10 mM (this drug is typically used at a final concentration of 5 µM to induce cell death)
? Coated 6 well tissue culture plates (1 - 2 mL volume of media is typically added to a well)
? Complete media

Opening Prompt: As part of a study into the effects of paclitaxel on MDA-MB-231 cells you are asked to set up a small experiment where cells are treated with the drug prior to their isolation to create lysates. You have the following materials. In addition to deciding on the treatments and controls you will have to work out the dilutions required. After you have had time to come up with a plan we will discuss as a group.

? Isolated MDA-MB-231 cell suspension (5 mL total) at a concentration of 463, 000 cells / mL
? Paclitaxel dissolved in DMSO at a stock concentration of 10 mM
? DMSO
? Apoptosis inducing DNA damage agent Doxorubicin dissolved in DMSO at concentration of 10 mM (this drug is typically used at a final concentration of 5 µM to induce cell death)
? Coated 6 well tissue culture plates (1 - 2 mL volume of media is typically added to a well)
? Complete media

Added by Joshua W.

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