2. Predict whether the GAL genes (Gal1, Gal7 Gal10 and Gal2) will be produced in the following yeast strains under the conditions noted. Use + to indicate that the GAL enzymes are synthesized at greater than basal levels, and - to indicate that the enzymes are not synthesized. NOTE: Gal4ts= a recessive temperature-sensitive allele of Gal4 Galactose absent Galactose present (a) wild-type (b) Gal4+/Gal4- (c) Gal3-/Gal3- (d) Gal80-/Gal80- (e) Gal4ts/ Gal4ts (at the permissive temperature) (f) Gal4ts/ Gal4ts (at the restrictive temperature) (g) Gal4+/ Gal4ts (at the restrictive temperature)
Added by Christopher T.
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(a) Wild-type yeast: GAL genes will be produced in the presence of galactose and not in its absence. Show more…
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Predict whether ̢-galactosidase (̢-gal) will be produced by the following E. coli strains under the conditions noted. The diploid genotypes represent F' (lac) strains. For each strain, use a plus sign (+) to indicate that ̢-gal is synthesized, and a minus sign (0) to indicate that the ̢-gal is not synthesized in both the absence and presence of lactose (list your answers in like this example - #11 0 and +). In this scenario, glucose is NOT present in the medium: Genetic Strain of E. coli Bacteria Lactose Absent Lactose Present 1. I+ P+ O+ Z+ 2. IS P+ O+ Z+ 3. I+ P+ OC Z+ 4. I- P+ OC Z+ 5. IS P+ O+ Z+/I+ P+ OC Z+ 6. I+ P+ OC Z-/I- P+ O+ Z+ 7. I+ P+ O+Z-/I- P+ O+ Z+ 8. I- P+ O+ Z-/I- P+ OC Z- 9. I+ P+ OC Z-/I- P+ O+ Z+ 10. IS P+ OC Z-/I+ P+ O+ Z+"
Sri K.
3. Predict whether functional ̢-galactosidase and Permease will be produced by the following E. coli strains under the conditions noted. The diploid genotypes represent F' (lac) strains. Use + to indicate that the enzyme is produced, and 0 to indicate that the enzyme is not produced. (8 pts)
Madhur L.
Match each genetic component involved with GAL gene regulation in yeast with the best description of its function. Enhancer element upstream of gal genes Transcription factor with a DNA binding domain and trans-activation domain Protein that interacts with GAL4 when no galactose is present Protein that senses the presence of galactose DNA binding protein that leads to repression of transcription in response to glucose
Derrick D.
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