Question 8 [15 marks / 15 minutes] Deamination of cytosine (C) gives uracil (U). a) Discuss how this chemical damage would be repaired. [4 marks] b) With respect to this DNA repair, propose what would be different if DNA normally had uracil instead of thymine (T). [4 marks] c) Explain when/how the chemical damage would become a mutation. [4 marks] d) Describe the mutation that could be produced. [3 marks]
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Deamination of cytosine (C) gives uracil (U). This chemical damage can be repaired through the base excision repair (BER) pathway. In this process, a DNA glycosylase enzyme recognizes and removes the uracil base, creating an abasic site. An AP endonuclease enzyme Show more…
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Deamination of cytosine (C) gives uracil (U). a) Discuss how this chemical damage would be repaired. b) With respect to this DNA repair, propose what would be different if DNA normally had uracil instead of thymine (T). c) Explain when/how the chemical damage would become a mutation. d) Describe the mutation that could be produced.
Sri K.
For Questions 1-5, state whether the mutation described is the result of: i. Base modifier ii. Base analogue iii. Intercalating agent iv. Ionizing radiation v. UV radiation vi. Tautomeric shift 1. Thymine-thymine dimer that leads to a base-substitution after DNA replication. 2. Base substitution (T→C) made by the polymerase mispairing the T (transiently in the rare enol form) with a G. 3. Translocation and a subsequent position effect that resulted from chromosome breaks. 4. Frameshift mutation due to the addition/insertion of one extra nucleotide. 5. Base substitution (C→T) resulting from an ingested chemical that deaminated a cytosine, changing it into uracil.
Ripujeet P.
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