Question 8: The DNA strand that mRNA is built from is called the template strand because it serves as a template for transcription. The mRNA molecules that are synthesized are complementary and antiparallel to the template strand of DNA and will contain uracil rather than thymine. The strand of DNA not used as a template for transcription is called the non-template strand. Sometimes the non-template strand is referred to as the ""coding strand"" because it corresponds to the same sequence as the resulting mRNA (which will likely go on to be translated into proteins). The only difference between the non-template strand and the new mRNA strand is instead of thymine, uracil takes its place in the mRNA strand. If the sequence of an mRNA molecule is: 5' AAGUCUACAGCU 3'. Answer the following questions: (a) What is the sequence of the template strand of DNA? (2 marks) The sequence of the template strand of DNA is 5' (b) What is the sequence of the nontemplate strand of DNA? (2 marks) The sequence of the nontemplate strand of DNA is 5' 3'. 3'. Please enter your sequences in the 5' to 3' direction. Deductions will be made if a sequence is inputted in the wrong direction. Carefully check that the sequence you input contains no typographical mistakes.
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Question 8: The DNA strand that mRNA is built from is called the template strand because it serves as a template for transcription. The mRNA molecules that are synthesized are complementary and antiparallel to the template strand of DNA and will contain uracil rather than thymine. The strand of DNA not used as a template for transcription is called the non-template strand. Sometimes the non-template strand is referred to as the "coding strand" because it corresponds to the same sequence as the resulting mRNA (which will likely go on to be translated into proteins). The only difference between the non-template strand and the new mRNA strand is instead of thymine, uracil takes its place in the mRNA strand. If the sequence of an mRNA molecule is: 5' AAGUCUACAGCU 3'. Answer the following questions: (a) What is the sequence of the template strand of DNA? (2 marks) The sequence of the template strand of DNA is 5' 3'. (b) What is the sequence of the nontemplate strand of DNA? (2 marks) The sequence of the nontemplate strand of DNA is 5' 3'. Please enter your sequences in the 5' to 3' direction. Deductions will be made if a sequence is inputted in the wrong direction. Carefully check that the sequence you input contains no typographical mistakes.
Farhan A.
A portion of the template strand of a gene is provided below: 5 ' - ATGACAAACGAT - 3 ' Answer the following questions about this DNA template. a. Provide the sequence of the portion of the mRNA that would be produced upon transcription of this DNA template. Provide single-letter nucleotide code in the 5' to 3' direction (do not use spaces or extra characters). 5'UACUGUUUGCU b. Assuming that translation begins starting from the first nucleotide, what amino acid sequence would be encoded by the portion of the mRNA transcribed in part (a)? Provide the single-letter amino acid code starting from the amino (N) terminus and proceeding toward the carboxyl (C) terminus (do not use spaces or extra characters). (N)YCLL c. What is the anticodon sequence from the tRNA that decodes the first codon in the portion of the mRNA that was transcribed in part (a)? Provide the anticodon sequence that forms three canonical (Watson-Crick) base pairs with the codon using single-letter nucleotide code in the 5' to 3' direction (do not use spaces or extra characters). 5'ACA3'
Sri K.
Transcription/Translation Homework The order of bases on the template strand of DNA in a gene is 3' AATACCTTGCAA 5’. This segment of DNA is from the beginning of a gene. (5 pts) What is the sequence of nucleotides in the other (complementary, sense) strand of DNA? Indicate which end is 3’ and which is 5’. What is the sequence of nucleotides in the mRNA? Indicate which end is 3’ and which is 5’. What are the first 3 amino acids this would code for? (Hint: begin with the start codon) If the first cytosine (C) in the template DNA strand is deleted, what is the sequence of nucleotides in the mRNA? What are the first 3 amino acids the mutated strand would code for? The order of bases on the template strand of DNA is 3' CCTACGGACGA 5'. This segment of DNA is from the beginning of a gene. (5 pts) a. What is the sequence of nucleotides in the other (complementary, sense) strand of DNA? Indicate which end is 3’ and which is 5’. b. What is the sequence of nucleotides in the mRNA? Indicate which end is 3’ and which is 5’. c. What are the first 3 amino acids this would code for? (Hint: begin with the start codon) d. If the first guanine (G) in the template DNA strand is deleted, what is the sequence of nucleotides in the mRNA? e. What are the first 3 amino acids the mutated strand would code for? NOTE: this is just the beginning of the sequence - assume the strand continues, even if you don't know the next letter.
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