QUESTIONS: 1. In the previous lab we used another red pH indicator, phenol red. Compare the colors of phenol red and methyl red at neutral and acidic conditions. 2. What is the difference between a selective medium and a differential medium? 3. Explain why Levine EMB is considered a selective medium but Luisetti's is not. 4. Use + or - to fill in this chart on the differences between E. coli and Enterobacter aerogenes Organism Indole Methyl Red Voges Proskauer Citrate E. coli Enterobacter aerogenes
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Comparing the colors of phenol red and methyl red at neutral and acidic conditions: Phenol red is yellow at acidic pH (below 6.8), red at neutral pH (6.8-8.2), and pink at alkaline pH (above 8.2). Methyl red is red at acidic pH (below 4.4), yellow at neutral pH Show more…
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In the previous lab we used another red pH indicator, phenol red. Compare the colors of phenol red and methyl red at neutral and acidic conditions. What is the difference between a selective medium and a differential medium? Explain why Levine EMB is considered a selective medium but Luisetti's is not. Use + or - to fill in this chart on the differences between E. coli and Enterobacter aerogenes. Organism Indole Methyl Red Voges Proskauer Citrate E. coli Enterobacter aerogenes
Madhur L.
Pre-Lab Questions 1. What does a functional group do in an organic molecule? 2. Account for the observation that many alcohols are water soluble. 3. Draw valid structures of (a) primary alcohol; (b) secondary alcohol; (c) tertiary alcohol; and (d) a phenol. In each example, circle the carbon-containing R groups. 4. Which of the compounds you show above in no. 4 would react with a base, such as NaOH? 5. Which color test from this experiment would be specific for a phenol?
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