Which of the following is NOT true about the sedimentation coefficient? A It is typically expressed in units of Svedbergs (S), with 1 Svedberg equal to $1 \times 10^{-13}$ seconds. B In order to calculate the sedimentation coefficient, you must know the radius of the centrifuge you are using. C A cellular component that is larger/denser than a second cellular component will have a smaller sedimentation coefficient than the second component. D To calculate the sedimentation coefficient for a given cellular component, you must measure the distances from the top of the liquid to the bottom of the tube and from the top of the pellet to the bottom of the tube after the centrifugation step that pellets the component of interest.
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Statement A: The sedimentation coefficient is typically expressed in Svedbergs (S), where 1 Svedberg is equal to $1 \times 10^{-13}$ seconds. This is true. Statement B: To calculate the sedimentation coefficient, you need to know the radius of the centrifuge. This Show more…
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Select all that apply. The sedimentation coefficient: is obtained when a particle has neutral buoyant density. is affected by the shape of the sedimenting particle. is directly proportional to the molecular weight of a particle in an ultracentrifuge. values are additive, so a 50S ribosome will dissociate to two 25S subunits. is expressed in Svedberg units, named after the scientist who invented the ultracentrifuge.
Madhur L.
Which of the following statements is false concerning the sedimentation coefficient (S) of a large molecule? Larger particle specific volumes tend to lower the sedimentation coefficient. Larger particle mass results in larger S values. Smaller and lighter molecules sediment faster during centrifugation through a dense fluid. The sedimentation coefficients of the parts of a molecular complex do not necessarily add up to that of the fully assembled complex. It is proportional to the rate of sedimentation during centrifugation through a dense fluid.
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