Why are you growing yeast on both a URA- and TRP- plate? Question 5 options: The yeast are not expected to grow on the TRP- plate because we disrupted the Trp gene using CRISPR/Cas9; however, you want to make sure the yeast can grow on a plate to ensure it is viable and be able to use it for future studies. The yeast are expected to grow on the TRP- and you want to be able to use it for future studies. Growth on URA- plate ensures that you have disrupted the Trp gene using CRISPR/Cas9. You want to double check your pipetting.
Added by Juan B.
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URA- plates lack uracil, which means that only yeast that can synthesize uracil will grow. TRP- plates lack tryptophan, so only yeast that can synthesize tryptophan will grow. Show more…
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